2,110
29
Essay, 2 pages (500 words)

Hemolysis of horse red blood cell

Hemolysis of Horse Red Blood Cell Practical Aim The results of this study will help show what solutes easily permeate a membrane. Further, this will help determine the factors affecting the rate of permeability of solutes.
Results
Isotonic Solution
Volume of Isotonic Solution (cm3)
Volume of Stock Solution (cm3)
Time
(min)
Sodium Chloride
10
1
30 (no hemolysis)
Ammonium Chloride
10
1
4
Ammonium Ethanoate
10
1
1
Ammonium Ethanodioate
10
1
4
Sodium Sulfate
10
1
33 (no hemolysis)
D-Glucose
10
1
35 (no hemolysis)
Glycerol
10
1
11
Ethanol
10
1
0. 8
Propano-1-ol
10
1
0. 9
Discussion/Conclusion
Hemolysis is the rupture of red blood cells when water enters the cell due to osmosis (Crawford). Osmosis is the movement of water from a higher water concentration to a lower water concentration. Thus, hemolysis will occur when a red blood cell is placed in a hypotonic medium, a medium that has a higher water concentration relative to that of the cytosol (Kimball).
Hemolysis will also happen if the cell is placed in an isotonic medium but the solute can pass through the cell membrane and the cell cannot remove these molecules that entered it (Ecklund, 4). This is shown in the above results. Despite that the cell was placed in an isotonic medium, 6 solutions caused hemolysis.
There are factors that contribute to the ability of the solute to pass through the cell membrane. One is the permeability of the cell membrane. Cell membrane is a lipid bilayer which does not allow ions and small hydrophilic molecules to freely pass the membrane (Kimball). Thus, for the sodium chloride, sodium sulfate and D-glucose solution to hemolyze the cell, a dilute solution should be used since this would increase the water concentration in the medium causing osmosis to occur.
Another factor that determine membrane permeability is lipid solubility. Generally, low solubility in lipid would mean slower rate of entry to the cell (Crawford). Hydrophilic substances will pass through the membrane slowly. As shown in the results, D-glucose, sodium chloride and sodium sulfate were not able to cause hemolysis,
Polarity and molecular size also affect the rate of entry of solute. Most polar molecules are hydrophilic; they cannot easily permeate the membrane (Cell Membrane, 2). Ethanol, propano-1-ol and glycerol, although polar, are small enough to pass through the membrane (Membrane Permeability). Notice that the time of hemolysis increases with molecular size with the glycerol having the longest time.
Ammonium chloride was able to hemolyze the cell compared to other salts. Unlike the other salts, ammonium chloride does not completely ionize in water. A lower degree of ionization would mean a faster rate of entry (Crawford).
Noting that glucose was not able to hemolyze the cell in 35 minutes, this shows that the cell absorbs glucose not simply through diffusion but using another mechanism.
Works Cited
Cell Membranes. University of Hawaii. 8 Dec 2006 Crawford, Kenneth M. Cell Membrane Permeability and Osmosis. 25 Aug. 2006. Western Kentucky University. 8 Dec 2006. Ecklund, P. R.. Osmosis and Membrane Permeability. 25 Sept. 2006. Cornell University. 8 Dec. 2006. Kimball, John W. Transport Across Cell Membranes. 29 Aug. 2006. 8 Dec 2006.
< http://home. comcast. net/john. kimball1/BiologyPages/D/Diffusion. html> Membrane Permeability. Andrews University. 8 Dec 2006. < http://www. biol. andrews. edu/
anat/anp1/lab/anp1. l6. html>

Thank's for Your Vote!
Hemolysis of horse red blood cell. Page 1
Hemolysis of horse red blood cell. Page 2
Hemolysis of horse red blood cell. Page 3
Hemolysis of horse red blood cell. Page 4
Hemolysis of horse red blood cell. Page 5

This work, titled "Hemolysis of horse red blood cell" was written and willingly shared by a fellow student. This sample can be utilized as a research and reference resource to aid in the writing of your own work. Any use of the work that does not include an appropriate citation is banned.

If you are the owner of this work and don’t want it to be published on AssignBuster, request its removal.

Request Removal
Cite this Essay

References

AssignBuster. (2021) 'Hemolysis of horse red blood cell'. 17 November.

Reference

AssignBuster. (2021, November 17). Hemolysis of horse red blood cell. Retrieved from https://assignbuster.com/hemolysis-of-horse-red-blood-cell/

References

AssignBuster. 2021. "Hemolysis of horse red blood cell." November 17, 2021. https://assignbuster.com/hemolysis-of-horse-red-blood-cell/.

1. AssignBuster. "Hemolysis of horse red blood cell." November 17, 2021. https://assignbuster.com/hemolysis-of-horse-red-blood-cell/.


Bibliography


AssignBuster. "Hemolysis of horse red blood cell." November 17, 2021. https://assignbuster.com/hemolysis-of-horse-red-blood-cell/.

Work Cited

"Hemolysis of horse red blood cell." AssignBuster, 17 Nov. 2021, assignbuster.com/hemolysis-of-horse-red-blood-cell/.

Get in Touch

Please, let us know if you have any ideas on improving Hemolysis of horse red blood cell, or our service. We will be happy to hear what you think: [email protected]